Factors affecting the toxicity of rotting carcasses containing Clostridium botulinum type E

Double Sandwich ELISA Modified Method for the Detection of Clostridium Botulinum Type E

Background & Objective: A very small amount of botulinum toxin can cause death and on the other hand, there is no cure for its poison other than antitoxin. Therefore, a diagnostic method that can detect very small amounts of botulinum toxin in a short time is very important. In this study, rapid and accurate detection of botulinum toxin type E has been performed with the double sandwich ELISA m...

Factors Affecting the Development of Clostridium Botulinum in Whole Milk.

This study was undertaken to determine the effect of the sterilization process and the age of the medium at the time of inoculation on the development of Clostridium botulinum type 62A. Whole milk was autoclaved at 121 C for 18 or 30 min and inoculated with C. botulinum so as to contain 2,000 to 5,000 spores per milliliter. No effort was made to remove dissolved oxygen or to reduce the oxidatio...

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Structure and toxicity of Clostridium botulinum type C toxin.

chromatography on a column of 9AE Sephadex A-50 in the presence of 5% of 2mercaptoethanol at OOC. No toxic activity was detected on both the chains. They were different from each other in antigenecity, amino acid composition and electric charge. Reassociation of these two components gave a molecule with iower texic act{vity (O.1-3% of the native toxin) in the delay of S-S linkage formation, whe...

Toxicity of Clostridium botulinum type E neurotoxin to Great Lakes fish: implications for avian botulism.

Since 1999, large-scale mortalities of fish-eating birds have been observed on the Great Lakes, and more specifically on Lake Erie. Type E botulism has been established as the primary cause of death. The mechanism of type E botulism exposure in fish-eating birds is unclear. Given that these birds are thought to eat live fish exclusively, it seems likely that their prey play a key role in the pr...